RSL1D1 polyclonal antibody
产品名称： RSL1D1 polyclonal antibody
英文名称： RSL1D1 polyclonal antibody
产品编号： PAB10306
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of RSL1D1.
- Immunogen:
- A synthetic peptide corresponding to internal region of human RSL1D1.
- Host:
- Rabbit
- Reactivity:
- Human
- Specificity:
- This antibody is specific to human PBK1 protein.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:2500-1:10000)
Western Blot (1:500-1:2000)
Immunoprecipitation (1:100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Identification of a novel nucleus protein involved in the regulation of urokinase in 95D cells.
Tong C, Tan L, Li P, Zhu YS.Acta Biochim Biophys Sin (Shanghai). 2005 May;37(5):303-9.
- 2.
- Suppression subtractive hybridization and expression profiling identifies a unique set of genes overexpressed in non-small-cell lung cancer.
Petroziello J, Yamane A, Westendorf L, Thompson M, McDonagh C, Cerveny C, Law CL, Wahl A, Carter P.Oncogene. 2004 Oct 7;23(46):7734-45.
- 3.
- Identification of differentially expressed genes in human trophoblast cells by differential-display RT-PCR.
Huch G, Hohn HP, Denker HW.Placenta. 1998 Nov;19(8):557-67.
- Applications
- Western Blot (Transfected lysate)
- Western blot using RSL1D1 polyclonal antibody (Cat # PAB10306) shows detection of over-expressed RSL1D1 in lysates from HeLa cells transfected with Flag-RSL1D1.
Lanes 1 and 3 contain lysate from Flag-RSL1D1 transfected HeLa cells.
Lanes 2 and 4 contain lysate from cells transfected with null vector.
Lanes 1 and 2 were blotted with anti-Flag antibody.
Lanes 3 and 4 were probed with a 1 : 500 dilution of RSL1D1 polyclonal antibody.
The band at 75 KDa, indicated by the arrowhead, corresponds to RSL1D1.
Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
- Immunofluorescence
- Immunofluorescence microscopy of HeLa cells transfected with RSL1D1 polyclonal antibody (Cat # PAB10306) .
In the overlay, specific antibody staining is shown to co-localize with recombinant protein.
Cells were fixed with methanol prior to staining.
Personal communication, J. McNally and D. Stavreva, NCI, Bethesda, MD.
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