COMT抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
COMT抗体

COMT抗体

商家询价

产品名称： COMT抗体

英文名称： COMTAntibody

产品编号： XY14754-1-

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司
  • 联系人 : 徐经理
  • 地址 : 上海市闵行莘庄工业区春东路508号A1-2F
  • 邮编 : 200612
  • 所在区域 : 上海
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 COMT抗体

Applications
In-house applications ELISA, IHC, IF, FC, IP, WB     Application not tested? Please see our Guarantee.
Published applications IHC, WB Publications 3 citations –view here
Species specificity Human,Mouse,Rat; other species not tested.
Published Species Human
Images
Prev12NextView more validation data
  • 14754-1-AP;H-liverImmunohistochemical of paraffin-embedded human liver using 14754-1-AP(COMT antibody) at dilution of 1:100 (under 10x lens)
  • 14754-1-AP;H-liverImmunohistochemical of paraffin-embedded human liver using 14754-1-AP(COMT antibody) at dilution of 1:100 (under 40x lens)
  • 14754-1-AP;HepG2Immunofluorescent analysis of HepG2 cells, using COMT antibody 14754-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
  • 14754-1-AP;mouse liver tissuemouse liver tissue were subjected to SDS PAGE followed by western blot with 14754-1-AP( COMT Antibody) at dilution of 1:600
  • 14754-1-AP;K-562 cellsIP Result of anti-COMT (IP:14754-1-AP, 4ug; Detection:14754-1-AP 1:500) with K-562 cells lysate 1200ug.
  • 14754-1-AP;HepG2 cells1X10^6 HepG2 cells were stained with 0.2ug COMT antibody (14754-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Positive WB detected in Mouse liver tissue, A431 cells, BxPC-3 cells
Positive IP detected in K-562 cells
Positive IHC detected in Human liver tissue
Positive IF detected in HepG2 cells
Positive FC detected in HepG2 cells
Recommended dilution WB : 1:200-1:2000
IP : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:20-1:200
COMT抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen COMT fusion protein ag6502 Full name Catechol-O-methyltransferase
Calculated molecular
weight		 30 kDa Observed molecular 
weight		 30 kDa
GenBank accession 
number		 BC000419 Gene ID (NCBI) 1312
Gene symbol COMT Synonyms Catechol O methyltransferase, COMT
BackgroundCOMT抗体

Catechol O-methyltransferase (COMT) is an important enzyme that catalyzes O-methylation and inactivation of metabolically active catechol-containing bioactive molecules and drugs. The mammalian COMT enzyme exists in soluble form (24-26 kDa) and membrane-bound form (28-30 kDa), which are encoded by a single gene utilizing different promoters.COMT抗体

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